| Rapid and Sensitive Detection of 3-Amino-2-oxazolidinone Using a Quantum Dot-Based Immunochromatographic Fluorescent Biosensor | |
Le, Tao1; Xie, Yong2 ; Zhu, Liqian3; Zhang, Lei1
| |
| 2016-11-16 | |
| 发表期刊 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| ISSN | 0021-8561 |
| EISSN | 1520-5118 |
| 卷号 | 64期号:45页码:8678-8683 |
| 摘要 | To monitor the levels of furazolidone in edible animal tissues, a fluorescent sensor was developed for the determination of 3-amino-2-oxazolidinone (AOZ), the metabolite of furazolidone, featuring an immunochromatographic test strip assay (ITSA) integrated with a quantum dot (QD) label. The optimal QD-based ITSA sensor exhibits good dynamic linear detection for AOZ over the range of 0.1-100 μg/L, with a 50% inhibitory concentration (IC50) of 1.06 μg/L. The decision limit and the detection capability were 0.14-0.15 and 0.27-0.33 μg/kg, respectively, for this analyte using the QD-based ITSA sensor. These values represent an improvement over a previously reported gold nanoparticle-based immunochromatographic assay. The recoveries of AOZ in kinds of animal tissues were between 76.3 and 98.4% at the levels of 1.0, 5.0, and 10.0 μg/kg. The performance and practicality of our QD-based fluorescent immunosensor were confirmed by commercial ELISA kit and LC-MS/MS. In conclusion, the proposed sensor was a feasible detection method for AOZ analysis on site. © 2016 American Chemical Society. |
| 关键词 | Animals Biosensors Fluorescence Gold nanoparticles Histology Metabolites Nanocrystals Tissue 3-amino-2-oxazolidinone Animal tissue Fluorescent immunosensors Furazolidone Immunochromatographic Immunochromatographic assays Inhibitory concentration Test strips |
| DOI | 10.1021/acs.jafc.6b03732 |
| 收录类别 | EI ; SCIE |
| 语种 | 英语 |
| WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
| WOS类目 | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
| WOS记录号 | WOS:000388430900026 |
| 出版者 | American Chemical Society |
| EI入藏号 | 20164803059130 |
| EI分类号 | 461.2 Biological Materials and Tissue Engineering ; 714.2 Semiconductor Devices and Integrated Circuits ; 741.1 Light/Optics ; 761 Nanotechnology |
| 原始文献类型 | Journal article (JA) |
| 出版地 | WASHINGTON |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | https://ir.cqcet.edu.cn/handle/39TD4454/2906 |
| 专题 | 数字媒体学院 |
| 作者单位 | 1.College of Life Science, Chongqing Normal University, Chongqing; 401331, China; 2.Chongqing College of Electronic Engineering, Chongqing; 401331, China; 3.College of Veterinary, Yangzhou University, Yangzhou; 225009, China |
| 推荐引用方式 GB/T 7714 | Le, Tao,Xie, Yong,Zhu, Liqian,et al. Rapid and Sensitive Detection of 3-Amino-2-oxazolidinone Using a Quantum Dot-Based Immunochromatographic Fluorescent Biosensor[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2016,64(45):8678-8683. |
| APA | Le, Tao,Xie, Yong,Zhu, Liqian,&Zhang, Lei.(2016).Rapid and Sensitive Detection of 3-Amino-2-oxazolidinone Using a Quantum Dot-Based Immunochromatographic Fluorescent Biosensor.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,64(45),8678-8683. |
| MLA | Le, Tao,et al."Rapid and Sensitive Detection of 3-Amino-2-oxazolidinone Using a Quantum Dot-Based Immunochromatographic Fluorescent Biosensor".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 64.45(2016):8678-8683. |
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