| Pressure-Based Immunoassays with Versatile Electronic Sensors for Carcinoembryonic Antigen Detection | |
| Huang, Lingting1; Zeng, Yongyi2; Liu, Xiaolong2; Tang, Dianping1,3 | |
| 2021-10-06 | |
| 发表期刊 | ACS Applied Materials & Interfaces
 |
| ISSN | 1944-8244 |
| EISSN | 1944-8252 |
| 卷号 | 13期号:39页码:46440-46450 |
| 摘要 | Pressure-based immunoassays have been studied for point-of-care testing for which increasing the sensitivity is still a challenge. In this study, we described an enhanced pressure-based immunoassay with a versatile electronic sensor for the sensitive biological analysis. The versatile electronic sensor had multifunctional sensing capabilities with temperature and pressure recording. Magnetic bead-modified capture antibody and platinum nanoparticle-labeled detection antibody were used as the biorecognition element of the target carcinoembryonic antigen (CEA) (as a model analyte) and would form a sandwich-type immune complex with CEA. After simple magnetic separation, this complex was transferred into the detection chamber, which contained both hydrogen peroxide (H2O2) and 3,3',5,5'-tetramethylbenzidine (TMB). With the catalytic ability of PtNPs, the H2O2-TMB-PtNPs system was catalyzed to generate a large amount of oxygen (O-2) and photothermal agent of oxidizer TMB (ox-TMB). Meanwhile, in a sealed chamber, further irradiation with an 808 nm near-infrared laser led to a triple-step signal amplification strategy of pressure increase, temperature increase, and gas thermal expansion to receive a strong electrical signal through the electronic sensor in real time. Thus, the amplified electrical signal from the electronic sensor could reveal the target concentration. In addition, we also verified that the synergistic system with two physical quantities had a lower limit of detection and a wider detection range compared to the detection system with a single physical quantity. In general, this immunoassay not only helped in exploring an effective signal amplification pathway but also offered an opportunity for the development of versatile electronic sensors in point-of-care settings. |
| 关键词 | versatile electronic sensor multimodal point-of-care testing pressure-based immunoassay carcinoembryonic antigen |
| DOI | 10.1021/acsami.1c16514 |
| 收录类别 | SCIE |
| 语种 | 英语 |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| WOS类目 | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| WOS记录号 | WOS:000706187100021 |
| 出版者 | AMER CHEMICAL SOC |
| 原始文献类型 | Article |
| 出版地 | WASHINGTON |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | https://ir.cqcet.edu.cn/handle/39TD4454/3748 |
| 专题 | 重庆电子科技职业大学 |
| 作者单位 | 1.Fuzhou Univ, Dept Chem, Key Lab Analyt Sci Food Safety & Biol MOE & Fujia, Fuzhou 350108, Peoples R China; 2.Fujian Med Univ, Mengchao Hepatobiliary Hosp, United Innovat Mengchao Hepatobiliary Technol Key, Fuzhou 350025, Peoples R China; 3.Chongqing Vocat Inst Engn, Chongqing 402260, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Lingting,Zeng, Yongyi,Liu, Xiaolong,et al. Pressure-Based Immunoassays with Versatile Electronic Sensors for Carcinoembryonic Antigen Detection[J]. ACS Applied Materials & Interfaces,2021,13(39):46440-46450. |
| APA | Huang, Lingting,Zeng, Yongyi,Liu, Xiaolong,&Tang, Dianping.(2021).Pressure-Based Immunoassays with Versatile Electronic Sensors for Carcinoembryonic Antigen Detection.ACS Applied Materials & Interfaces,13(39),46440-46450. |
| MLA | Huang, Lingting,et al."Pressure-Based Immunoassays with Versatile Electronic Sensors for Carcinoembryonic Antigen Detection".ACS Applied Materials & Interfaces 13.39(2021):46440-46450. |
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