| Electrochemical detection of lipopolysaccharide based on rolling circle amplification assisted formation of copper nanoparticles for enhanced resistance generation | |
| Xie, Shunbi1; Zhang, Jin2; Teng, Liumei1; Yuan, Wei1; Tang, Ying1; Peng, Qin1; Tang, Qinchun3 | |
| 2019-12-12 | |
| 发表期刊 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| EISSN | 0925-4005 |
| 卷号 | 301 |
| 摘要 | In this work, a novel sandwich-type impedimetric biosensor was fabricated for highly sensitive and selective detection of lipopolysaccharide (LPS) from E. coli O55:B5. LPS aptamer I was immobilized onto electrodeposited gold nanoparticles (Au) modified electrode for capturing LPS in buffer and serum. LPS aptamer II and rolling circle amplification (RCA) primer were conjugated with gold nanoparticles (AuNPs) to use as signal tags. LPS was sandwiched between AuNPs/primer/aptamer II bioconjugates and aptamer I. This was followed by extension of RCA, which resulted in long poly-thyrnine (T) single strand (ssDNA) sequences to adsorb copper ions (Cu2+) into the skeleton of poly-T sequences. Thus, poly-T ssDNA directly served as the specific template for copper nanoparticles (CuNPs) formation with the aid of ascorbic acid (VC). Finally, the formed CuNPs@ssDNA complex promoted the oxidation of pyrocatechol violet (PCV) to insoluble bulky oligomer (precipitation) in the presence of H2O2. The reaction significantly enhanced the resistance signal of LPS quantitative determination. The impedimetric aptasensor exhibited a wide dynamic working range of 0.01 pg/mL(similar to) 100 ng/mL with a detection limit of 4.8 fg/mL. This method also showed good selectivity and reproducibility, and demonstrated the applicability of LPS detection in human serum samples, offering a promising avenue for sensitive LPS detection. |
| 关键词 | Sandwich-type impedimetric aptasensor Lipopolysaccharide Rolling circle amplification Copper nanoparticles Precipitation |
| DOI | 10.1016/j.snb.2019.127072 |
| 收录类别 | SCIE |
| 语种 | 英语 |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| WOS类目 | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
| WOS记录号 | WOS:000489529300033 |
| 出版者 | ELSEVIER SCIENCE SA |
| 原始文献类型 | Article |
| 出版地 | LAUSANNE |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | https://ir.cqcet.edu.cn/handle/39TD4454/3629 |
| 专题 | 重庆电子科技职业大学 |
| 作者单位 | 1.Chongqing Univ Arts & Sci, Chongqing Key Lab Environm Mat & Remediat Technol, Chongqing 402160, Peoples R China; 2.Chongqing Vocat Inst Engn, Chongqing 402260, Peoples R China; 3.Hosp Tradit Chinese Med Yongchuan, Chongqing 402160, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Shunbi,Zhang, Jin,Teng, Liumei,et al. Electrochemical detection of lipopolysaccharide based on rolling circle amplification assisted formation of copper nanoparticles for enhanced resistance generation[J]. SENSORS AND ACTUATORS B-CHEMICAL,2019,301. |
| APA | Xie, Shunbi.,Zhang, Jin.,Teng, Liumei.,Yuan, Wei.,Tang, Ying.,...&Tang, Qinchun.(2019).Electrochemical detection of lipopolysaccharide based on rolling circle amplification assisted formation of copper nanoparticles for enhanced resistance generation.SENSORS AND ACTUATORS B-CHEMICAL,301. |
| MLA | Xie, Shunbi,et al."Electrochemical detection of lipopolysaccharide based on rolling circle amplification assisted formation of copper nanoparticles for enhanced resistance generation".SENSORS AND ACTUATORS B-CHEMICAL 301(2019). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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