An immunochromatography test strip for rapid, quantitative and sensitive detection of furazolidone metabolite, 3-amino-2-oxazolidinone, in animal tissues

doi:10.1080/09540105.2017.1293013

CQUET-IR

	An immunochromatography test strip for rapid, quantitative and sensitive detection of furazolidone metabolite, 3-amino-2-oxazolidinone, in animal tissues
	Xie, Yong 1; Zhang, Lei 2; Le, Tao 2
	2017-05
发表期刊	FOOD AND AGRICULTURAL IMMUNOLOGY
ISSN	0954-0105
EISSN	1465-3443
卷号	28 期号:3 页码:403-413
摘要	3-amino-2-oxazolidinone (AOZ) is the metabolite of furazolidone, which has been banned as a veterinary drug due to the potential harmful effects on human health. In this study, a novel immunochromatography test strip (ICTS) based on colloidal gold-McAb probe for detection of AOZ within tissue samples was developed. With this method, the visual cut-off levels for the test strip was achieved at 10g/L. Using a test strip reader, the 50% inhibition was 1.3g/L. The limit of detection and limit of quantification in four kinds of animal tissues were 0.15g/kg and 0.31g/kg, respectively. When used to analyze fortified samples of animal tissue, acceptable recovery rates of 76.3-98.4% were obtained. The fortified samples were detected by ICTS and confirmed by LC-MS/MS. The results of the two methods were in good agreement. The proposed ICTS was demonstrated to be a rapid and simple analytical method for detecting AOZ in animal tissue.
关键词	Furazolidone 3-amino-2-oxazolidinone immunochromatography test strip colloidal gold nanoparticles
DOI	10.1080/09540105.2017.1293013
收录类别	SCIE
语种	英语
WOS研究方向	Chemistry ; Food Science & Technology ; Immunology ; Toxicology
WOS类目	Chemistry, Applied ; Food Science & Technology ; Immunology ; Toxicology
WOS记录号	WOS:000400172800004
出版者	TAYLOR & FRANCIS LTD
原始文献类型	Article
出版地	ABINGDON
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.cqcet.edu.cn/handle/39TD4454/3551
专题	重庆电子科技职业大学
作者单位	1.Chongqing Coll Elect Engn, Chongqing, Peoples R China; 2.Chongqing Normal Univ, Coll Life Sci, Chongqing 401331, Peoples R China
推荐引用方式 GB/T 7714	Xie, Yong,Zhang, Lei,Le, Tao. An immunochromatography test strip for rapid, quantitative and sensitive detection of furazolidone metabolite, 3-amino-2-oxazolidinone, in animal tissues[J]. FOOD AND AGRICULTURAL IMMUNOLOGY,2017,28(3):403-413.
APA	Xie, Yong,Zhang, Lei,&Le, Tao.(2017).An immunochromatography test strip for rapid, quantitative and sensitive detection of furazolidone metabolite, 3-amino-2-oxazolidinone, in animal tissues.FOOD AND AGRICULTURAL IMMUNOLOGY,28(3),403-413.
MLA	Xie, Yong,et al."An immunochromatography test strip for rapid, quantitative and sensitive detection of furazolidone metabolite, 3-amino-2-oxazolidinone, in animal tissues".FOOD AND AGRICULTURAL IMMUNOLOGY 28.3(2017):403-413.