Immunochromatographic Assay for Simultaneous and Quantitative Detection of 3-Methyl-Quinoxaline-2-Carboxylic Acid and Quinoxaline-2-Carboxylic Acid Residues in Animal Tissues Based on Highly Luminescent Quantum Dot Beads

doi:10.1007/s12161-017-1003-2

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	Immunochromatographic Assay for Simultaneous and Quantitative Detection of 3-Methyl-Quinoxaline-2-Carboxylic Acid and Quinoxaline-2-Carboxylic Acid Residues in Animal Tissues Based on Highly Luminescent Quantum Dot Beads
	Xie, Yong1,2 ; Zhang, Lei 1; Sun, Qi 1; Zhang, Zhihao 1; Le, Tao 1
	2018-02-01
发表期刊	Food Analytical Methods
ISSN	1936-9751
EISSN	1936-976X
卷号	11 期号:2 页码:334-342
摘要	Due to their potential adverse effects on human health, the use of carbadox and olaquindox in feedingstuffs was prohibited by the European Union since 1998. In this work, highly luminescent quantum dot beads (QBs) were synthesized by encapsulating CdSe/ZnS and used as novel fluorescent probes in the immunochromatographic assay (ICA) for simultaneous and quantitative determination of metabolites of olaquindox (3-methylquinoxaline-2-carboxylic acid, MQCA) and carbadox (quinoxaline-2-carboxylic, QCA). The fluorescence intensities of the test lines were recorded using a fluorescence strip reader. The 50% of inhibition for MQCA and QCA was shown to be 8.1 and 10.6 μg L−1, respectively. The whole assay process can be accomplished within 10 min. The immunosensor was used to analyze spiked samples, and analyte intra- and inter-assay recovery rates ranged from 78.7 to 92.2% for MQCA and 80.6 to 95.8% for QCA, and coefficients of variation were all below 12%. The incurred tissues samples were assayed using both QB-based ICA and commercial ELISA kit and were further confirmed with LC-MS/MS. The QB-based ICA results exhibited good agreement with both commercial ELISA and LC-MS/MS methods. © 2017, Springer Science+Business Media, LLC.
关键词	Cadmium compounds Carboxylic acids Fluorescence Histology II VI semiconductors Magnetic logic devices Metabolites Nanocrystals Tissue Coefficients of variations Fluorescence intensities Fluorescent probes Fluorescent sensors Immunochromatographic assays Quantitative detection Quantitative determinations Quinoxaline-2-carboxylic acids
DOI	10.1007/s12161-017-1003-2
收录类别	EI ; SCIE
语种	英语
WOS研究方向	Food Science & Technology
WOS类目	Food Science & Technology
WOS记录号	WOS:000419178200003
出版者	Springer Science and Business Media, LLC
EI入藏号	20173104009034
EI分类号	461.2 Biological Materials and Tissue Engineering ; 714.2 Semiconductor Devices and Integrated Circuits ; 721.2 Logic Elements ; 741.1 Light/Optics ; 761 Nanotechnology ; 804.1 Organic Compounds
原始文献类型	Journal article (JA)
出版地	NEW YORK
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.cqcet.edu.cn/handle/39TD4454/3164
专题	数字媒体学院
作者单位	1.College of Life Science, Chongqing Normal University, 37 Chengzhong Road, University Town, Chongqing; 401331, China; 2.Chongqing College of Electronic Engineering, Chongqing; 401331, China
第一作者单位	重庆电子科技职业大学
推荐引用方式 GB/T 7714	Xie, Yong,Zhang, Lei,Sun, Qi,et al. Immunochromatographic Assay for Simultaneous and Quantitative Detection of 3-Methyl-Quinoxaline-2-Carboxylic Acid and Quinoxaline-2-Carboxylic Acid Residues in Animal Tissues Based on Highly Luminescent Quantum Dot Beads[J]. Food Analytical Methods,2018,11(2):334-342.
APA	Xie, Yong,Zhang, Lei,Sun, Qi,Zhang, Zhihao,&Le, Tao.(2018).Immunochromatographic Assay for Simultaneous and Quantitative Detection of 3-Methyl-Quinoxaline-2-Carboxylic Acid and Quinoxaline-2-Carboxylic Acid Residues in Animal Tissues Based on Highly Luminescent Quantum Dot Beads.Food Analytical Methods,11(2),334-342.
MLA	Xie, Yong,et al."Immunochromatographic Assay for Simultaneous and Quantitative Detection of 3-Methyl-Quinoxaline-2-Carboxylic Acid and Quinoxaline-2-Carboxylic Acid Residues in Animal Tissues Based on Highly Luminescent Quantum Dot Beads".Food Analytical Methods 11.2(2018):334-342.

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