Selection and truncation of aptamers as fluorescence sensing platforms for selective and sensitive detection of nitrofurazone

doi:10.1016/j.aca.2023.341044

CQUET-IR > 数字媒体学院

	Selection and truncation of aptamers as fluorescence sensing platforms for selective and sensitive detection of nitrofurazone
	Wen, Xu 1; Yan, Xueling 1; Zheng, Xiaoling 1; Kou, Qiming 1; Yang, Lulan 1; Tang, Jiaming 1; Chen, Xingyue 1; Xie, Yong2 ; Le, Tao 1
	2023-04-29
发表期刊	ANALYTICA CHIMICA ACTA
ISSN	0003-2670
EISSN	1873-4324
卷号	1252
摘要	Nitrofurazone (NFZ) is an antibiotic banned in many countries, as its residue seriously harms the human body. Herein, anti-NFZ aptamers were selected and identified based on the magnetic bead SELEX technique using a ssDNA library with a full length of 90 nucleotides (nt). Five full sequence candidate aptamers (NFZ8, NFZ24, NFZ28, NFZ34, and NFZ70) were obtained by secondary structure analysis. We optimized the entire sequence to obtain a truncated aptamer, a 16 nt sequence (NFZ8-1:5′-GTTCTATTGAAAAAAC-3′) that showed the highest affinity for NFZ (Kd = 76.11 nM). The binding site of NFZ and aptamer NFZ8-1 was found to be "GAA" by molecular docking. In addition, utilizing the most special truncated aptamer NFZ8-1 as the identification probe, a graphene oxide fluorescent aptasensor is an innovative for the detection of NFZ residue that showed a wide linear reach from 1.25 to 160 ng/mL and a low limit of detection of 1.13 ng/mL. In the actual water environment sample detection, the recovery rate ranged from 95.21 to 113.66%, and the coefficient of variation ranged from 3.53 to 11.24%. These results demonstrate that the NFZ-truncated aptamer applied to the aptasensor provides a novel methodology for recognizing NFZ residues. © 2023
关键词	Graphene Molecular modeling Aptamer truncation Aptamers Aptasensors Fluorescence sensing Fluorescent aptasensor Magnetic bead SELEX Magnetic beads Molecular docking Nitrofurazone Sensing platforms
DOI	10.1016/j.aca.2023.341044
收录类别	EI ; SCIE
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000957765300001
出版者	Elsevier B.V.
EI入藏号	20231113738509
原始文献类型	Journal article (JA)
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.cqcet.edu.cn/handle/39TD4454/17736
专题	数字媒体学院
作者单位	1.College of Life Science, Chongqing Normal University, Chongqing; 401331, China; 2.Bioassay 3D Reconstruction Laboratory, Chongqing College of Electronic Engineering, Chongqing; 401331, China
推荐引用方式 GB/T 7714	Wen, Xu,Yan, Xueling,Zheng, Xiaoling,et al. Selection and truncation of aptamers as fluorescence sensing platforms for selective and sensitive detection of nitrofurazone[J]. ANALYTICA CHIMICA ACTA,2023,1252.
APA	Wen, Xu.,Yan, Xueling.,Zheng, Xiaoling.,Kou, Qiming.,Yang, Lulan.,...&Le, Tao.(2023).Selection and truncation of aptamers as fluorescence sensing platforms for selective and sensitive detection of nitrofurazone.ANALYTICA CHIMICA ACTA,1252.
MLA	Wen, Xu,et al."Selection and truncation of aptamers as fluorescence sensing platforms for selective and sensitive detection of nitrofurazone".ANALYTICA CHIMICA ACTA 1252(2023).