Au Nanoparticle-Decorated ZnO Microflower-Based Immunoassay for Photoelectrochemical Detection of Human Prostate-Specific Antigen

doi:10.1021/acsanm.1c02397

CQUET-IR

	Au Nanoparticle-Decorated ZnO Microflower-Based Immunoassay for Photoelectrochemical Detection of Human Prostate-Specific Antigen
	Xu, JH ; Li, YX ; Gong, HX ; Yu, ZC ; Zhang, J ; Wei, QH ; Tang, DP
	2021-10-22
发表期刊	ACS APPLIED NANO MATERIALS
EISSN	2574-0970
卷号	4 期号:10 页码:10943-10951
摘要	Herein, an in situ amplified photoelectrochemical (PEC) immunoassay with ZnO microflowers (ZnO MFs) decorated with gold nanoparticles (Au NPs) was developed to determine human prostate-specific antigen (PSA) using L-cysteine-loaded liposomes for signal amplification. Initially, ZnO MFs with smooth and well-defined morphology were synthesized under hydrothermal conditions. The heterostructured microflowers were formed by depositing Au NPs on ZnO microflowers using trisodium citrate. L-Cysteine (L-Cys)-encapsulated liposomes conjugated with detection antibodies were used to fabricate a sandwiched immunocomplex on a capture antibody-modified microtiter plate in the presence of target PSA. The liposomes were lysed using Triton X-100 to release the encapsulated L-Cys, thereby increasing the photocurrent on Au NP-decorated ZnO MFs. Results indicated that the photoelectrochemical immunoassay displayed good photocurrents to response PSA concentrations from 0.01 to 20 ng mL(-1), and the detection PSA concentration was as low as 0.79 pg mL(-1). Furthermore, the photoelectrochemical immunoassay had good precision, high selectivity, and well-matched accuracy toward target PSA in human serum specimens using the commercialized human PSA ELISA kit as a reference.
关键词	ZnO microflowers SOLAR-CELLS gold nanoparticles ARRAYS nanohybrid materials HETEROJUNCTION photoelectrochemical immunoassay ABSORPTION l-cysteine-loaded liposome NANOTUBES prostate-specific antigen EMISSION PLATFORM FIELD
DOI	10.1021/acsanm.1c02397
收录类别	SCIE
语种	英语
WOS研究方向	Science & Technology - Other Topics ; Materials Science
WOS类目	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号	WOS:000711030600098
出版者	AMER CHEMICAL SOC
原始文献类型	Article
出版地	WASHINGTON
引用统计	被引频次：28[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.cqcet.edu.cn/handle/39TD4454/10415
专题	重庆电子科技职业大学
作者单位	1.Fuzhou Univ, Dept Chem, Key Lab Analyt Sci Food Safety & Biol, MOE & Fujian Prov, Fuzhou 350108, Peoples R China; 2.Chongqing Vocat Inst Engn, Chongqing 402260, Peoples R China
推荐引用方式 GB/T 7714	Xu, JH,Li, YX,Gong, HX,et al. Au Nanoparticle-Decorated ZnO Microflower-Based Immunoassay for Photoelectrochemical Detection of Human Prostate-Specific Antigen[J]. ACS APPLIED NANO MATERIALS,2021,4(10):10943-10951.
APA	Xu, JH.,Li, YX.,Gong, HX.,Yu, ZC.,Zhang, J.,...&Tang, DP.(2021).Au Nanoparticle-Decorated ZnO Microflower-Based Immunoassay for Photoelectrochemical Detection of Human Prostate-Specific Antigen.ACS APPLIED NANO MATERIALS,4(10),10943-10951.
MLA	Xu, JH,et al."Au Nanoparticle-Decorated ZnO Microflower-Based Immunoassay for Photoelectrochemical Detection of Human Prostate-Specific Antigen".ACS APPLIED NANO MATERIALS 4.10(2021):10943-10951.