Click Chemistry Reaction-Triggered 3D DNA Walking Machine for Sensitive Electrochemical Detection of Copper Ion

doi:10.1021/acs.analchem.8b02555

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	Click Chemistry Reaction-Triggered 3D DNA Walking Machine for Sensitive Electrochemical Detection of Copper Ion
	Qing, Min 1,2; Xie, Shunbi 1; Cai, Wei 1,2; Tang, Dianyong 1; Tang, Ying 1; Zhang, Jin 3; Yuan, Ruo 2
	2018-10-02
发表期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
EISSN	1520-6882
卷号	90 期号:19 页码:11439-11445
摘要	Herein, for the first time, we engineered click chemistry reaction to trigger a 3D DNA walking machine for ultrasensitive electrochemical detection of copper ion (Cu2+), which provided a convenient access to overcome the shortcomings of poor selectivity and limited amplification efficiency in traditional determination of Cu2+. Click chemistry reaction drove azido-S2 to bind with alkynyl-S1 for the formation of a walker probe on aminated magnetic polystyrene microsphere@gold nanoparticles (PSC@Au), which opened the hairpin-locked DNAzyme. In the presence of magnesium ion (Mg2+), the unlocked DNAzyme was activated to cleave the self-strand at the facing ribonucleotide site, accompanied by the release of product DNA (S3) and the walker probe. Therefore, the walker probe was able to open the adjacent hairpin-locked DNAzyme strand and then be released by DNAzyme cleavage along the PSC@Au-DNAzyme track. Eventually, the liberated single-strand S3 induced catalytic hairpin assembly (CHA) recycling, resulting in the capture of a large number of methylene blue-tagged hairpin DNA (MB-H2) on the sensor surface and significant electrochemical responses. By coupling click chemistry reaction with the dual-amplification strategy of the 3D DNA walking machine and CHA recycling, the proposed biosensor not only demonstrated high accuracy and selectivity for Cu(2+)detection in real samples but also showed excellent performance for Cu2+ detection with a wide linear range of 1.0 pM to 500 nM and low detection limit of 0.33 pM. Moreover, this elaborated biosensor could be readily expanded to Mg2+ detection with a constant concentration of Cu2+, which paves a new way to apply the 3D DNA walking machine in various ion sensings.
DOI	10.1021/acs.analchem.8b02555
收录类别	SCIE
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000446542600039
出版者	AMER CHEMICAL SOC
原始文献类型	Article
出版地	WASHINGTON
引用统计	被引频次：83[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.cqcet.edu.cn/handle/39TD4454/3663
专题	重庆电子科技职业大学
作者单位	1.Chongqing Univ Arts & Sci, Chongqing Key Lab Environm Mat & Remediat Technol, Chongqing 402160, Peoples R China; 2.Southwest Univ, Coll Chem & Chem Engn, Minist Educ, Key Lab Luminescent & Real Time Analyt Chem, Chongqing 400715, Peoples R China; 3.Chongqing Vocat Inst Engn, Chongqing 402260, Peoples R China
推荐引用方式 GB/T 7714	Qing, Min,Xie, Shunbi,Cai, Wei,et al. Click Chemistry Reaction-Triggered 3D DNA Walking Machine for Sensitive Electrochemical Detection of Copper Ion[J]. ANALYTICAL CHEMISTRY,2018,90(19):11439-11445.
APA	Qing, Min.,Xie, Shunbi.,Cai, Wei.,Tang, Dianyong.,Tang, Ying.,...&Yuan, Ruo.(2018).Click Chemistry Reaction-Triggered 3D DNA Walking Machine for Sensitive Electrochemical Detection of Copper Ion.ANALYTICAL CHEMISTRY,90(19),11439-11445.
MLA	Qing, Min,et al."Click Chemistry Reaction-Triggered 3D DNA Walking Machine for Sensitive Electrochemical Detection of Copper Ion".ANALYTICAL CHEMISTRY 90.19(2018):11439-11445.

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