Development of a quantum dot-based immunochromatography test strip for rapid screening of oxytetracycline and 4-epi-oxytetracycline in edible animal tissues

doi:10.1080/19440049.2016.1277038

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	Development of a quantum dot-based immunochromatography test strip for rapid screening of oxytetracycline and 4-epi-oxytetracycline in edible animal tissues
	Xie, Yong1,2 ; Zhang, Lei 1; Yang, Xian 1; Le, Tao 1
	2017-03-04
发表期刊	Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
ISSN	1944-0049
EISSN	1944-0057
卷号	34 期号:3 页码:371-378
摘要	A rapid and sensitive immunochromatographic test strip (ICTS) was developed in a competitive format with the quantum dot-conjugated monoclonal antibody specifically to determine the residues of oxytetracycline (OTC) and its metabolite 4-epi-oxytetracycline (4-epi-OTC). Using an ICTS reader, the 50% inhibition concentration (IC50) was found to be 3.41 ± 0.29 ng ml–1 of OTC in phosphate-buffered saline samples and the detection limit was 0.44 ± 0.05 ng ml–1. The visual cut-off level of the ICTS is 25 ng ml–1. The recoveries from tissue samples spiked with OTC of 50–600 μg kg–1 were 74.2–107.2%, with coefficients of variation below 15%. The method was used to analyse incurred tissue samples, and there was good correlation (R2 = 0.999) between the ICTS and high-performance liquid chromatography. These results indicate that ICTS is suitable for the rapid and quantitative detection of OTC and 4-epi-OTC residues in edible animal tissues. © 2017 Informa UK Limited, trading as Taylor & Francis Group.
关键词	Animals High performance liquid chromatography Histology Metabolites Monoclonal antibodies Nanocrystals Semiconductor quantum dots Tissue 4-epi-Oxytetracycline Coefficients of variations Immunochromatographic Inhibition concentrations Oxytetracyclines (OTC) Phosphate-buffered salines Quantitative detection Test strips
DOI	10.1080/19440049.2016.1277038
收录类别	EI ; SCIE
语种	英语
WOS研究方向	Chemistry ; Food Science & Technology ; Toxicology
WOS类目	Chemistry, Applied ; Food Science & Technology ; Toxicology
WOS记录号	WOS:000398080500005
出版者	Taylor and Francis Ltd.
EI入藏号	20170503297031
EI分类号	461 Bioengineering and Biology ; 714.2 Semiconductor Devices and Integrated Circuits ; 761 Nanotechnology
原始文献类型	Journal article (JA)
出版地	ABINGDON
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.cqcet.edu.cn/handle/39TD4454/3028
专题	数字媒体学院
作者单位	1.College of Life Science, Chongqing Normal University, Chongqing, China; 2.Department of Animation, Chongqing College of Electronic Engineering, Chongqing, China
第一作者单位	重庆电子科技职业大学
推荐引用方式 GB/T 7714	Xie, Yong,Zhang, Lei,Yang, Xian,et al. Development of a quantum dot-based immunochromatography test strip for rapid screening of oxytetracycline and 4-epi-oxytetracycline in edible animal tissues[J]. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment,2017,34(3):371-378.
APA	Xie, Yong,Zhang, Lei,Yang, Xian,&Le, Tao.(2017).Development of a quantum dot-based immunochromatography test strip for rapid screening of oxytetracycline and 4-epi-oxytetracycline in edible animal tissues.Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment,34(3),371-378.
MLA	Xie, Yong,et al."Development of a quantum dot-based immunochromatography test strip for rapid screening of oxytetracycline and 4-epi-oxytetracycline in edible animal tissues".Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment 34.3(2017):371-378.