| An AuNPs-Based Fluorescent Sensor with Truncated Aptamer for Detection of Sulfaquinoxaline in Water | |
| Chen, Xingyue1; Yang, Lulan1; Tang, Jiaming1; Wen, Xu1; Zheng, Xiaoling1; Chen, Lingling1; Li, Jiaqi1; Xie, Yong2; Le, Tao1 | |
| 2022-07 | |
| 发表期刊 | BIOSENSORS-BASEL
 |
| EISSN | 2079-6374 |
| 卷号 | 12期号:7 |
| 摘要 | Herein, we developed a novel truncation technique for aptamer sequences to fabricate highly sensitive aptasensors based on molecular docking and molecular dynamics simulations. The binding mechanism and energy composition of the aptamer/sulfaquinoxaline (SQX) complexes were investigated. We successfully obtained a new SQX-specific aptamer (SBA28-1: CCCTAGGGG) with high affinity (K-d = 27.36 nM) and high specificity determined using graphene oxide. This aptamer has a unique stem-loop structure that can bind to SQX. Then, we fabricated a fluorescence aptasensor based on SBA28-1, gold nanoparticles (AuNPs), and rhodamine B (RhoB) that presented a good linear range of 1.25-160 ng/mL and a limit of detection of 1.04 ng/mL. When used to analyze water samples, the aptasensor presented acceptable recovery rates of 93.1-100.1% and coefficients of variation (CVs) of 2.2-10.2%. In conclusion, the fluorescence aptasensor can accurately and sensitively detect SQX in water samples and has good application prospects. |
| 关键词 | molecular docking molecular dynamics simulations aptamer truncation fluorescence aptasensor gold nanoparticles |
| DOI | 10.3390/bios12070513 |
| 收录类别 | SCIE |
| 语种 | 英语 |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation |
| WOS类目 | Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation |
| WOS记录号 | WOS:000831485900001 |
| 出版者 | MDPI |
| 原始文献类型 | Article ; 期刊论文 |
| 出版地 | BASEL |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | https://ir.cqcet.edu.cn/handle/39TD4454/13928 |
| 专题 | 重庆电子科技职业大学 |
| 作者单位 | 1.Chongqing Normal Univ, Coll Life Sci, Chongqing 401331, Peoples R China; 2.Chongqing Coll Elect Engn, Bioassay 3D Reconstruct Lab, Chongqing 401331, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Xingyue,Yang, Lulan,Tang, Jiaming,et al. An AuNPs-Based Fluorescent Sensor with Truncated Aptamer for Detection of Sulfaquinoxaline in Water[J]. BIOSENSORS-BASEL,2022,12(7). |
| APA | Chen, Xingyue.,Yang, Lulan.,Tang, Jiaming.,Wen, Xu.,Zheng, Xiaoling.,...&Le, Tao.(2022).An AuNPs-Based Fluorescent Sensor with Truncated Aptamer for Detection of Sulfaquinoxaline in Water.BIOSENSORS-BASEL,12(7). |
| MLA | Chen, Xingyue,et al."An AuNPs-Based Fluorescent Sensor with Truncated Aptamer for Detection of Sulfaquinoxaline in Water".BIOSENSORS-BASEL 12.7(2022). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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